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KMID : 0861020130280060039
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Korea Journal of Herbology
2013 Volume.28 No. 6 p.39 ~ p.45
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Histological analysis of five organs in streptozotocin-induced diabetic rats
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Oh Tae-Woo
Kang Seok-Yong
Park Yong-Ki
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Abstract
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Objectives : This study was conduct to compare of histological changes on four target organs which related with diabetes in streptozotocin-induced diabetic rats.
Methods : Diabetes was induced in male Sprague-Dawley rats by consecutive injection of streptozotocin (STZ) at different doses of 30, 40 and 50 ㎎/㎏ for 5 days. After 4 weeks, all rats were sacrificed, five different organs such as pancreas, liver, kidney, and lung were isolated and observed their histological changes by hematoxylin and eosin (H&E), Periodic acid-Schiff (PAS) and Masson`s trichrome staining. The changes of body weight, blood glucose, and food and water intake were also measured.
Results : The multiple administration of STZ was induced diabetes in rats with hyperglycemia, decrease of body weight, increase water and food intake, and histopathological changes of target organs, compared with those of normal rats in both dose-dependent and time-dependent manner. In histological analysis, pancreas was showed decrease of the islet numbers with beta-cell loss. Kidney showed morphological damage with glomerulus hypertrophy, and also lung was showed bronchial epithelial damage with inflammatory cells infiltration. In liver, the portal vein and hepatic artery could not observed, and showed inflammatory cell infiltration with liver fibrosis.
Conclusions : These results suggest that the increase of the capacity of STZ, each of the more chronic disease, it can be seen that the damage was deep. Thus, evaluate the resulting drug appropriate depending on the purpose of the model is expected to be selected.
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KEYWORD
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Diabetes, Streptozotocin, multiple administration, pancrease, kidney, lung, liver, histopathological analysis
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